CD8A and neoplasm: Tumor nests contained too few CD8+ T cells to perform a reliable analysis, but assuming that CD8+ T cells localized within the tumor and within 20 μm from the tumor could mediate cytotoxic killing of malignant cells, we combined the tumor and the stromal area 20 μm from the tumor into one annotation to analyze the number of CD8+ T cells with putative capacity to interact with tumor cells (Figure 5D).