Moreover, we confirmed that the mRNA and protein levels of NXPH4 were higher in bladder cancer cell lines (BIU-87, 5637, RT112, and T24) than in the normal bladder cell line SV-HUC-1 by qRT-PCR and WB (Figure 3D,E). The gene discussed is NXPH4; the disease is urinary bladder carcinoma.