In order to address the contribution of CD44 to GBM progression, we performed RNA-sequencing of either U251MG KO cells (clone #28) or parental cells in the presence or absence of the γ-secretase inhibitor DAPT, which is known to inhibit CD44 cleavage; the CD44 cleavage product CD44-ICD has been implicated in mediating CD44-dependent transcriptional responses [30]. Here, CD44 is linked to glioblastoma.