For example, RNF20-depleted human MCF10A non-malignant epithelial cells and mouse NIH-3T3 fibroblasts showed higher oncogenic potential in various oncogenic cell behavior tests, suggesting that RNF20 is a tumor suppressor [150], while in laminal breast cancer cells RNF20 silencing reduced proliferation, migration, and tumorigenic and metastatic capacity, indicating its tumor promoting role [216]. This evidence concerns the gene RNF20 and breast carcinoma.