To investigate the involvement of M1-linked linear ubiquitination in TDP-43 proteinopathy, we first constructed GFP-tagged full-length and truncated forms of the ALS-associated A315T mutant of TDP-43 (Figure 1A), and ectopically expressed them in mouse neuroblastoma Neuro2a cells. The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.