TARDBP and amyotrophic lateral sclerosis: In this study, we showed that cytoplasmic aggregates of ectopically expressed, truncated ALS-associated TDP-43 mutants in Neuro2a cells contained M1-, K48-, and K63-ubiquitins in vitro, and the MG-132-mediated inhibition of proteasome activity enhanced the ubiquitination of TDP-43 (Figure 1, Figure 2 and Figure 3).