A nested PCR targeting the tpi and gdh gene targets was useful and helped to confirm issues in the the pet prairie dogs in the current study, such as infection with zoonotic species of G. duodenalis, assemblage A and B. G. duodenalis assemblages A and B have been most frequently reported in humans as well as in cattle, dogs, and cats from different parts of the world [5]. This evidence concerns the gene TPI1 and infection.