To further investigate the function of HK2 on regulating migratory capacity in human ovarian cancer cells, exogenous HK2 was stably overexpressed in SKOV3 (SKOV3-GFP and SKOV3-HK2, Fig. 1A) cells; conversely, endogenous expression of HK2 was knocked down by stably transfecting shRNA plasmids in A2780 (A2780-shCtr and A2780-shHK2, Fig. 1D) cells. Here, HK2 is linked to ovarian carcinoma.