CD4 and neoplasm: The proportion of various immune cells in the homogenates were measured by flow cytometry shown in Additional file 1: Fig. S5, including tumor infiltrating lymphocytes (TILs, CD45+), CD8+ T cells, CD4+ T cells, myeloid-derived suppressor cells (MDSCs, Gr-1+CD11b+), tumor-associated M2 macrophages (M2-polarized TAMs, CD206+F4/80+) and regulatory T cells (Tregs, FOXP3+CD4+).