Consistently, Vγ9Vδ2 T-cells, immuno-purified from PBMC and co-cultured in normoxic conditions with cancer cells, pre-conditioned by normoxia, hypoxia, hypoxia/normoxia and intermittent hypoxia (Fig. 4e), were progressively less proliferating and cytolytic, as indicated by the lower percentage of Ki67+IFNγ+ Vγ9Vδ2 T-cells (Fig. 4f) and by the lower amount of necro-apoptotic (i.e., annexin V+/PI+) cancer cells (Fig. 4g). The gene discussed is MKI67; the disease is cancer.