Liquid chromatography with tandem mass spectrometry (LC–MS/MS) analyses of tRNA isolated from non-infected and CHIKV-infected cells, as well as from non-infected cells overexpressing a myc-DDK-tagged-KIAA1456 protein (Fig. 5b), showed that both CHIKV infection and KIAA1456 overexpression induced a significant increase in the levels of mcm5 modification (CHIKV-infection: 1.3-fold change, p = 0.011, two-tailed t-test; KIAA1456: 1.4-fold change, p = 0.016, two-tailed t-test) (Fig. 5c). This evidence concerns the gene TRMT9B and infection.