To address the SP1-77 neutralization mechanism, we applied a lattice light sheet microscopy (LLSM) approach to monitor target cell infection by a chimeric vesicular stomatitis virus in which the coat glycoprotein was replaced by the SARS-CoV-2 (Wuhan strain) S protein (VSV-SARS-CoV-2) (Fig. 6 and Fig. S12) (52). Here, SP1 is linked to infection.