SIAH1 and neoplasm: To verify this hypothesis in a preclinical animal model, we developed an in vivo tumour model (Figure 7A) We subcutaneously and intraperitoneally injected A2780 cells transfected with Vector, SIAH1, Clt-shRNA or sh-SIAH1 lentivirus and A2780 cells in which RPS3 is stably knocked down transfected with SIAH1+FLAG-RPS3 or SIAH1+FLAG-RPS3 (K214R) lentivirus into female BALB/c nude mice with the aim of producing appropriately sized tumours (n = 6).