ITGA4 and muscle tissue disorder: As shown in Tables 2 and 3, the nodes (proteins) in the PPI network (Fig. 1) participate in pathways like actin filament-based movement, muscle contraction, cytoskeleton organization, muscle organ development, cardiomyopathy, ITGA4, ITGAV, and NCKAP1, which are all found in the 2q31.3–q32.2 chromosomal region, where the candidate gene for DA10 is thought to lie, do not appear to be viable candidates for DA10 because they are not seen in skeletal or muscular disorders to any significant level [12].