INS and hydrops fetalis: At D63, plasma insulin and leptin concentrations at D63 were significantly higher in HF-SED group as compared with the Chow-SED or HF-EEX group (P = 0·07 and < 0·001, and F value (df 39, 39) = 2·729 and (df 39, 39) = 16·79, respectively) (Fig. 2(c)).