Combined with Yang’s research [23], we conclude that miR-26a-5p suppresses proliferation, invasion and migration, promotes apoptosis though inhibiting Epithelial-mesenchymal transition (EMT) by targeting HSDL2 in the CC cells, which supplemented the mechanism of miR-26a-5p-HSDL2-EMT on influencing CC progression. The gene discussed is HSDL2; the disease is cholangiocarcinoma.