Unexpectedly, proliferation, invasion and migration were inappreciably reduced; apoptosis was slightly increased in the Hela and C33A cells with miR-NC + OE-HSDL2, relative to cell with miR-NC + OE-NC, which seemed inconsistent with the upregulation of HSDL2 in CC patients. The gene discussed is HSDL2; the disease is cholangiocarcinoma.