Nevertheless, the rescue experiments showed overexpression of HSDL2 partly reversed the inhibition of proliferation, invasion and migration, and stimulation of apoptosis induced by miR-26a-5p, which demonstrated that the miR-26a-5p retarded proliferation, invasion and migration, and accelerated apoptosis via regulating HSDL2 in the CC cells. This evidence concerns the gene HSDL2 and cholangiocarcinoma.