To validate the observation in prostate cancer, we vaccinated mice with epitopes derived from PSMA and identified three Th1 selective epitopes that secreted significantly higher IFN-γ than IL-10 (p466 (p < 0.0001), p570 (p = 0.06) and p582 (p < 0.0001)) and three epitopes (p181, p216, and p397 (all p < 0.002)) that primarily induced IL-10 secretion as compared to IFN-γ (Fig. 2A). This evidence concerns the gene IL10 and prostate cancer.