The HURP-gap was not a by-product of the GFP-tag or cell line since it was also visible after staining with antibodies directed against endogenous HURP (Fig. 1G) and visible in live images of HeLa (hypertriploid cervical carcinoma) cells expressing endogenously tagged EGFP-HURP (Fig. 1H and Supplementary Movie 3), as well as fixed HCT-116 (pseudo-diploid colorectal carcinoma) and ECRF24 (immortalised human umbilical vein endothelial) cells stained with HURP antibodies (Fig. 1I). Here, DLGAP5 is linked to cervical carcinoma.