TARDBP and amyotrophic lateral sclerosis: We first examined differential gene expression between control and TDP-43-knockdown samples [human HeLa cells (Roczniak-Ferguson and Ferguson, 2019), human SH-SY5Y cells (Melamed et al., 2019), ihMNs (Klim et al., 2019), mouse mammary gland (Zhao et al., 2020), mouse striatum (Polymenidou et al., 2011) and rat primary astrocytes (LaRocca et al., 2019)], and ALS/FTD TDP-43-immunopositive (TDP pos) and TDP-43-immunonegative (TDP neg) neuronal nuclei (Liu et al., 2019), using the DESeq2 package.