As TDP-43 is known to regulate splicing and cryptic exon expression (Ling et al., 2015; Tollervey et al., 2011), we next examined differential exon usage (DEU) between control and TDP-43-knockdown samples, and ALS/FTD TDP pos and TDP neg neuronal nuclei, using the DEXSeq package. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.