Figure 4b shows that STAT2 protein could specifically bind at Region 1 (highlighted in dark blue) and Region 2 (highlighted in light blue), confirming the physical interaction between STAT2 and PD-L1 promoter. Furthermore, we obtained the RNA-seq data of colon cancer cells with STAT2-knockdown from the GEO dataset GSE137155. PD-L1 expression was significantly downregulated in the STAT2-knockdown group (figure 4c). This evidence concerns the gene CD274 and malignant colon neoplasm.