Of the top 20 most significant diet DEGs identified in the CC, carbamoyl-phosphate synthase 1 (Cps1), isovaleryl-CoA dehydrogenase (Ivd), neuropilin 1 (Nrp1), and pyruvate kinase L/R (Pklr) were previously found to be associated with obesity traits in humans [38, 51, 69, 72, 108], but only one of the top 20 most significant strain DEGs was associated with at least one obesity trait in humans, namely glycerophosphodiester phosphodiesterase domain containing 3 (Gdpd3) [108]. This evidence concerns the gene PKLR and obesity due to melanocortin 4 receptor deficiency.