Co-embedding together with DNA methylation profiles from a previously reported cohort of 43 histologically low-grade gliomas arising in children with NF1 [7] demonstrated that the majority of those tumors clustered with the 18 molecular low-grade NF1-asociated gliomas from our study rather than the 3 reference subclasses of sporadic pilocytic astrocytoma (Supplementary Fig. 6 [Online Resource 2]). Here, NF1 is linked to central nervous system cancer.