Using CRISPR-DiR in an AML and a hepatocellular carcinoma cell line, the authors were able to identify a specific region in the 5 ́-UTR CpG island that—upon demethylation—upregulated SALL4. Interestingly, treatment of these cell lines with decitabine led to comparable demethylation at this CpG island and a dose-dependent increase of SALL4 expression. This evidence concerns the gene SALL4 and hepatocellular carcinoma.