HES1 and neoplasm: We obtained tumor tissues from Keap1 mutant, wild-type, and Nrf2 mutant patients’ samples and immunostained them for the expression of Notch1, Hes1, Ki67, and Nrf2. Immunostaining results confirmed the absence of Nrf2 in Nrf2 mutant tumor tissues and high levels of Keap1 mutant tumors (Fig. 6E).