Further, out of the eight dysregulated proteins detected across all time points in the 5XFAD mice (Figs. 1E and 6A), five proteins (APP, apolipoprotein E, glial fibrillary acidic protein, clusterin, and integral membrane protein 2B) are also defined as AD biomarkers in DisGeNET’s AD biomarker database. Here, APP is linked to Alzheimer disease.