The fact that IGF1 and Wnt3a induced a variable response in other CRC cell lines, as shown here, indicates that mutations in PIK3CA, APC, and CTNNB1, as is the case in HT-29 and HCT-116 cells,29,30 are necessary for the ligand-mediated potentiating effect to occur. This evidence concerns the gene IGF1 and colorectal carcinoma.