In addition, the results of gene and protein expression confirmed the upregulation of these three NOXes in the L-NAME group; thus, our experimental model of hypertension by nitric oxide deprivation presented with significant increases in the mRNA and protein expression of NOX1, NOX2, and NOX4 (~ 2–2.5-fold; Fig. 3D–F). Here, CYBB is linked to hypertensive disorder.