To find out, whether AGO2-ex1/3 can be translated from this second start codon referring to M78 in melanoma cells, we generated an expression plasmid with the complete AGO2-ex1/3 mRNA sequence containing an additional internal HA sequence in exon6 (pAGO2-ex1/3HAint, Fig. 2G). This evidence concerns the gene AGO2 and melanoma.