This study aimed to detect the expression of HDAC9 and TLR4 in different human populations followed by ox-LDL incited atherosclerosis macrophages cell line to detect whether HDAC9 could regulate macrophage polarization along with inflammatory response through TLR4 regulation, which is envisaged to provide a new idea for the treatment of the targets of atherosclerosis. The gene discussed is HDAC9; the disease is atherosclerosis.