To obtain an accurate overview of changes occurring during fibrocyte-to-CAF differentiation in ESCC, we isolated circulating FGFR2+ fibrocytes and their paired FGFR2+ CAFs from 9 ESCC patients and compared their gene expression profiles by high-throughput RNA-seq (Gene Expression Omnibus accession number GSE103111). Here, FGFR2 is linked to esophageal squamous cell carcinoma.