According to the prediction results of the over-representation analysis enrichment method, we obtained 59 pairs of TF-IRG interactions, including 9 TFs (NFAT, COUP, STAT4, TEF1, P53, PPAR, TATA, FREAC2, PU1) and 24 IRGs, of which 7 IRGs (TNFRSF19, TGFB2, GREM1, SPP1, PGF, INHBB, and GRP) were upregulated and 17 IRGs (SEMA6D, BMP5, TPM2, SCG2, NRG1, FABP2, ANGPTL1, POMC, UCN3, COLEC12, RBP2, PTH1R, CCL15, AGTR1, ACVRL1, NTS, and CCL28) were downregulated in CRC. The gene discussed is PGF; the disease is colorectal carcinoma.