To understand the role of prostate cancer exosomes in macrophage polarization, we first induced THP1 cells into macrophages with 10 ng/ml PMA for 72 h [20], and then these macrophages were treated with IFN-γ (50 ng/mL), IL-4 (25 ng/mL), 2B4-exos (100 μg/ml), and IE8-exos (100 μg/ml) [21]. Here, IL4 is linked to Familial prostate cancer.