CETN2 and breast cancer: Similar to depletion of MyoJ, Cen2, or actin, which all cause defects in final BC constriction and likely the formation of the posterior cup10,13,14, BCC1-depleted parasites appeared ‘stumpy’ due to loss of basal tapering and presented a ~30% wider basal diameter of the mature IMC due to lack of BC constriction (Fig. 8f).