Cas12a was used to generate several animal models for human diseases, such as atherosclerosis in rats (by multiplex knock-out of the Apoe and Ldlr genes) [51], Werner syndrome in rabbits [20], Duchenne muscular dystrophy (DMD) [20], and cryopyrin-associated periodic syndrome (CAPS) [53] in pigs. Here, NLRP3 is linked to Duchenne muscular dystrophy.