Thereafter, peripheral blood mononuclear cells (PBMCs) isolated from two subjects who had been treated 6 months earlier, (one with dermatitis and one without dermatitis), were cultured in the presence of different concentrations of benznidazole, either pure or derived from pills of the commercial product or with RPMI supplemented with dimethylsulfoxid (DMSO) for 40 hours and analyzed for interferon-γ (IFN-γ) production (Fig. 2A to C). Here, IFNG is linked to dermatitis.