Shen et al. found a significant range of PD-L1 expression in osteosarcoma specimens, with 6 of 38 (15.8%) samples having negative expression, 4 of 38 (10.5%) samples with low PD-L1 expression, 19 of 38 (38%) with intermediate PD-L1 expression, and 9 of 38 (23.7%) samples with high PD-L1 expression [30]. Here, CD274 is linked to osteosarcoma.