Regarding the osteoporosis pathological phenotypes in femur tissue sections, the H&E staining and histomorphology analyses revealed decreased bone mass with loss of trabecular bone underneath growth plates as well as increased marrow adipocytes with enhanced adipogenic potential, and decreased osteogenic differentiation potential in NTCP-KO OVX mice compared with WT OVX mice (Figure 4A). This evidence concerns the gene SLC10A1 and osteoporosis.