C3 and neoplasm: Indeed, C3a was detected in the supernatant of Pan02 cells by ELISA (Supplementary Fig. S3b) and using the LDH release assay, we demonstrated that these NK cells possess cytotoxic activity against Pan02 cells at ratio of NK cells to tumor cells ranging from 1.25:1 to 20:1 (Supplementary Fig. S3c).