In this study, we analyzed the expression characteristics of TLR1 to TLR 10 in various cancer tissues using a variety of bioinformatics methods, comprehensively analyzed TLRs, and found that the transcriptional levels of TLRs were associated with stemness, tumor purity, and drug sensitivity in cancer tissues included in The Cancer Genome Atlas (TCGA). The gene discussed is TLR1; the disease is cancer.