It was found that high-TMB group had more CD8 T cells in LUSC and CESC (p=0.008, p=0.012), less CD4 memory resting T cells in LUSC (p=0.004), more CD4 memory activated T cells in LUSC and CESC (p=0.014, p=0.030), more follicular helper T cells in LUSC (p=0.012), less regulatory T cells in ESCC, LUSC and CESC (p=0.024, p=0.011, p=0.025), more resting NK cells in HNSCC (p=0.047), more activated NK cells in LUSC (p=0.006), less monocytes in ESCC (p=0.044), more macrophages M1 in LUSC and CESC (p<0.001, p=0.010). The gene discussed is CD4; the disease is esophageal squamous cell carcinoma.