High prevalence rates of microvascular complications were detected even in young adults with T1DM (James et al., 2014), which would increase the difficulty of subject recruitment and management; 4) moreover, prescribed medicine for comorbidity sometimes may affect the assessment of study insulin; 5) an additional run-in period of up to 6 h is usually required before clamping for the normalization of BG (Porcellati et al., 2019; Linnebjerg et al., 2020a; Heise et al., 2020). This evidence concerns the gene INS and type 1 diabetes mellitus.