In OC cells and tumor tissues, we found that the expression of tumor suppressor genes P53 and PTEN was activated in puerarin-treated groups, along with the altered expression of other related genes, including P21, BRCA1, BIRC5, and CTGF. This indicates that puerarin has the potential to activate the expression of tumor suppressor genes, thus contributing to the inhibition of OC cell survival and proliferation and induction of apoptosis. Here, TP53 is linked to neoplasm.