We divided these patients into six groups: KMT2A group, ANKRD11 group, EP300 group, SETD5 group, AFF4 group and a remaining group consisted of a lower number of patients with features of CdLS caused by non-cohesion genes variants (BRD4, ARID1B, SMARCB1, TAF1, DDX23, CSNK1G1, ZMYND11, MED13L, PHIP, TAF6, NAA50, CREBBP, UBE2A, KMT2D, and KDM6A). The gene discussed is AFF4; the disease is Cornelia de Lange syndrome.