Subsequently, NOX4, senescence markers, and inflammation-related factors were also highly expressed, being highest in the group loaded with AOPPs after the puncture; the results of GAGS content measured by DMMB and DHI measured by X-ray were lowest in the group loaded with AOPPs after the puncture, suggesting that an increase in AOPPs in the disc accelerates disc degeneration. Here, NOX4 is linked to intervertebral disk degenerative disorder.