MLKL and colon carcinoma: We previously reported that CRISPR/Cas9-mediated knockout (KO) of USP22 in the human colon carcinoma cell line HT-29 affects RIPK3 ubiquitination during necroptosis without inducing major changes in RIPK1, RIPK3, and MLKL gene expression [53], suggesting gene-specific regulation of USP22.