To investigate the [Ca2+]ex-induced IL-1β response in obesity, macrophages were generated from monocytes from healthy individuals who did not have obesity and from people with obesity in vitro in the presence of autologous serum in order to recapitulate the metabolic conditions in vivo in the blood and in AT (Supplementary Fig. 2). The gene discussed is IL1B; the disease is obesity due to melanocortin 4 receptor deficiency.