In conclusion, the present study confirms that zerumbone is a promising candidate for the anticancer therapy due to strong inhibitory and apoptotic effects on pediatric rhabdomyosarcoma cell lines through increased caspase-3/7 activity and increased ROS production as well as through the modulation of NF-κB pathway and merit further investigation including in vivo analysis. The gene discussed is NFKB1; the disease is rhabdomyosarcoma.