In contrast to subcutaneous tumours, anti-CTLA-4treatment failed to deplete Foxp3+ Tregs in orthotopic tumours (Fig. 5G, and Supplementary Fig. 8F).Furthermore, the majority of CD4+ T cells in subcutaneous tumourswere Tregs whilst in orthotopic tumours CD4+ effector T cells weremore abundant (SupplementaryFig. 8G). The gene discussed is CD4; the disease is neoplasm.