We have investigated the impact of partial genetic deletion of Tgfb1 and Smad3 on the expression of genes involved in TGFβ (Tgfb1, Pai1) and BMP (Bmp2, Alk2) signaling and osteoblast differentiation (Spp1, Runx2) during CAVD development and progression in Kl–/– mice. This evidence concerns the gene TGFB1 and congenital bilateral aplasia of vas deferens from CFTR mutation.