We have investigated the impact of partial genetic deletion of Tgfb1 and Smad3 on the expression of genes involved in TGFβ (Tgfb1, Pai1) and BMP (Bmp2, Alk2) signaling and osteoblast differentiation (Spp1, Runx2) during CAVD development and progression in Kl–/– mice. Here, RUNX2 is linked to congenital bilateral aplasia of vas deferens from CFTR mutation.