Interestingly, we reconstituted plasmid of expressed Flag-tagged YWHAG(s) by the synonymous mutation and transfected Flag-tagged YWHAG(s) and empty vector into YWHAG-KO cells or control cells, following infection with PR8 MOI = 0.01 at 12–36 h p.i. The result of TCID50 assays demonstrated significantly reduced after transfection of Flag-tagged YWHAG(T) into the YWHAG-KO cells compared with empty vector into the YWHAG-KO cells (Supplementary Figure 4). The gene discussed is YWHAG; the disease is infection.