Furthermore, ELISA assay showed that knockdown of AHCY decreased the secretion of IL-1β and IL-6 in the supernatant of SW620 cells (Figures 6(e) and 6(f)), and AHCY overexpression increased the secretion of IL-1β and IL-6 in the supernatant of DLD-1 cells (Figures 6(g) and 6(h)), highlighting that AHCY positively regulated inflammation in colon cancer cells. The gene discussed is IL6; the disease is malignant colon neoplasm.